Free Ebook Next-Generation Sequencing Data Analysis

[PDF.DN7j] Next-Generation Sequencing Data Analysis

[PDF.DN7j] Next-Generation Sequencing Data Analysis

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A Practical Guide to the Highly Dynamic Area of Massively Parallel Sequencing The development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphism, genome structure, and epigenomic abnormality. Next-Generation Sequencing Data Analysis shows how next-generation sequencing (NGS) technologies are applied to transform nearly all aspects of biological research. The book walks readers through the multiple stages of NGS data generation and analysis in an easy-to-follow fashion. It covers every step in each stage, from the planning stage of experimental design, sample processing, sequencing strategy formulation, the early stage of base calling, reads quality check and data preprocessing to the intermediate stage of mapping reads to a reference genome and normalization to more advanced stages specific to each application. All major applications of NGS are covered, including: RNA-seq: mRNA-seq and small RNA-seq Genotyping and variant discovery through genome re-sequencing De novo genome assembly ChIP-seq to study DNAprotein interaction Methylated DNA sequencing on epigenetic regulation Metagenome analysis through community genome shotgun sequencing Before detailing the analytic steps for each of these applications, the book presents the ins and outs of the most widely used NGS platforms, with side-by-side comparisons of key technical aspects. This helps practitioners decide which platform to use for a particular project. The book also offers a perspective on the development of DNA sequencing technologies, from Sanger to future-generation sequencing technologies. The book discusses concepts and principles that underlie each analytic step, along with software tools for implementation. It highlights key features of the tools while omitting tedious details to provide an easy-to-follow guide for practitioners in life sciences, bioinformatics, and biostatistics. In addition, references to detailed descriptions of the tools are given for further reading if needed. The accompanying website for the book provides step-by-step, real-world examples of how to apply the tools covered in the text to research projects. All the tools are freely available to academic users. DNA Sequencing Costs: Data - National Human Genome DNA Sequencing Costs: Data Data from the NHGRI Genome Sequencing Program (GSP) Overview For many years the National Human Genome Research Institute (NHGRI) has Partek Inc NGS & microarray data analysis software Statistical and visual data analysis software Widely used in life sciences and engineering for gene expression (microarray) data analysis high throughput screening Next-generation DNA sequencing : Article : Nature Second-generation DNA sequencing Alternative strategies for DNA sequencing can be grouped into several categories (as discussed previously in ref 4) A comparison of tools for the simulation of genomic next The next-generation sequencing (NGS) simulation process begins with the input of a reference sequence (in most cases) and simulation parameters Next Generation Dx Summit Next Generation Dx Summit 2017 Next Generation Dx Summit convenes more than 1000 international diagnostic professionals for valuable networking and comprehensive Next-Generation Sequencing (NGS) Explore the technology Find the Right Next-Generation Sequencing Platform Our interactive NGS platform selection tool can help you find the right sequencer for your needs An Introduction to Next-Generation Sequencing Technology An Introduction to Next-Generation Sequencing Technology illuminacom/technology/next-generation-sequencinghtml Next-generation Sequencing: Current Technologies and With the introduction of next generation sequencing (NGS) technologies in 2005 the domination of microarrays in genomics quickly came to an end due to NGS' superior Next Generation Sequencing - Genomics and Sequencing Center MiSeq NGS; Sample Library Preparation; Amplicon Library Preparation and Analysis; MiSeq Data Flow; MiSeq NGS Introduction For two decades Sanger sequencing has Next Generation Sequencing (NGS) Services NGS Services Send us your samples and let our Next Generation Sequencing (NGS) experts perform your NGS analysis in state-of-the-art automated laboratories with
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